Experimental study of A66G-single nucleotide polymorphism in the MTRR gene and acute myeloid leukemia

Abstract

Acute Myeloid leukemia (AML) is the most common leukemia among adult population which begins with the bone marrow, but it frequently spreads to the blood stream as well. One of the common polymorphisms in AML is A66G in Methionine Synthase Reductase (MTRR), which converts isoleucine into methionine residue in the protein chain, the methionine/homocysteine cycle is disrupted. Limited studies were documented between A66G polymorphism in MTRR gene with AML. This study aimed to investigate the A66G polymorphism in MTRR gene with AML patients in the Saudi population. Peripheral blood was collected from 100 AML patients and 100 controls and DNA was isolated. Using A66G primers, polymerase chain reaction was performed followed by restriction fragment length polymorphism analysis. The mean age of both cases and controls was found to be an average of 39 years. Allele (G vs A: OR-3.41 [95 %CI: 1.87–11.24; p = 0.0001) and genotype analysis (GG vs AA: OR-3.43 [95 %CI: 1.45–8.11]; p = 0.0004) has shown the association. In conclusion, A66G polymorphism has a strong genetic role in the AML patients in Saudi population.