Abstract
ABSTRACT When Drosophila eggs are centrifuged at 4250 g posteriorly, anteriorly and laterally, the highest mortality rate was found among the eggs centrifuged posteriorly (46 %) and the smallest among those centrifuged laterally (20 %). The pole plasm is not displaced by centrifugation at 4250 g. Polar granules begin to move when centrifuged at 2700 g for 30 min; complete removal of granules takes place at 4250 g for 10 min. When polar granules are removed from the pole plasm no pole cells are detached from the egg. Polar granules shifted from the pole plasm to other regions do not induce the detachment of any cells similar to pole cells. Both pole plasm and polar granules, in association, are indispensable for pole cell formation in Drosophila melanogaster.
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