Experimental studies on the regulatory effect of rhTNF-α on thymidine phosphorylase and effects of rhTNF-α on inhibiting effect on cervical cancer SiHa cells by Capecitabine

Abstract

Objective To investigate the regulatory effect of recombination human tumor necrosis factor-α (rhTNF-α) on thymidine phosphorylase and effect of rhTNF-α on inhibiting effect of capectiabine on cervical cancer SiHa cells. Methods The SiHa human cervical cancer cell line cultured in vitro were given different concentrations of rhTNF-α; and the cell growth inhibiting ratio of SiHa cells were assessed by MTT assay, the level of thymidine phsohphorylase was detected by enzyme linked immunosorbent assay (ELISA). Results rhTNF-α can significantly inhibit the growth of SiHa human cervical cancer cells with obvious amount-effect relationship and time-effect relationship; and the level of TP increased gradually with the increase of the level of rhTNF-α, the difference was statistically significant (F=26.87, P<0.01); rhTNF-α and Capecitabine had obvious synergy, and had a dosage-dependent relationship, there was a significantly difference between the groups which received medication and control group (F=30.56, P<0.05). Conclusions rhTNF-α could actively suppress SiHa cells proliferation in vitro, and can cause a concertration-dependent increase in the level of TP, and enhance anticancer effect of Capecitabine on cervical cancer cells. Key words: Recombination human tumor necrosis factor-α; SiHa cells; Thymidine Phsohphorylase; Capecitabine