Experimental Studies Concerning the Effects of Administration of Sesame Oil Emulsion on the Anterior Pituitary Ovarian Functions of the Pregnant and Non-Pregnant Rats

Abstract

Sesame oil emulsion was administered intra-peritoneally, at the rate of 1 cc. (0.2 gm.) per 100 gm. body weight, for ten successive days to the non-pregnant (diestrus and estrus) and pregnant (early and late stages) Wister strain rats.The results were as follows : 1. There were no significant changes concerning the pituitary and ovarian weights in any group.2. Total cholesterol content in serum was appearently reduced in the non-pregnant rats, while there was no significant change in the pregnant rats. No detectable changes were observed regarding the contents of total fatty acids and phospholipids in serum in any of the non-pregnant and pregnant rats.3. Total cholesterol content in ovaries was clearly elevated in the non-pregnant rats, while there was no significant change in the pregnant rats. No statistically meaningful changes were observed concerning the contents of total fatty acids and phospholipids in ovaries in any group.4. Regarding the pituitary gonadotropic potency, there was a slight rise in the non-pregnant rats, while there were no significant changes in the pregnant rats.5. An increase of the number of beta cells in the anterior pituitary and the degranulation of beta granules were obvious in the non-pregnant rats. These changes were not observed in any of the pregnant rats.6. Sudanophilic droplets in ovaries became more coarse and numerous in both the non-pregnant and the pregnant group. However, these changes were more evident in the non-pregnant groups.7. Cyclic changes of vaginal smears became slightly irregular, but no quiescence of cyclic changes was recognized in the non-pregnant rats. The changes of glycogen content index of vaginal smears were nearly in parallel with the changes of vaginal cornification index. In the pregnant grougs, however, these changes were not observed.These results lead to the following conclusions : In the non-pregnant rats, in contrast with the normal cyclic hormonal changes of the non-treated rats, the administration of sesame oil emulsion elicited an interesting change in the features of the anterior pituitary ovarian axis. There were enough evidences to show that the anterior pituitary of the treated, produced and secreted more gonadotropin than that of the non-treated ; however, the ovaries were not influenced by the changes of the anterior pituitary.The attitude of the reacting ovaries was rather quiet in character causing deposition and increace of cholesterol content-the material of steroidogenesis.These changes which were elicited by the treatments of sesame oil emulsion in the non-pregnant rats were not observed in the pregnant rats.