Abstract
The capability of Streptococcus iniae to cause disease in Japanese flounder was investigated by oral and bath (30 min) challenges with low, medium and high inoculation doses. Only with the highest inoculation dose, 9.9t107 CFU/100 g body weight, the oral challenge induced deaths. In the bath challenge deaths were induced even at the lowest inoculation dose, 2.9t103 CFU/mL water. Hemorrhagic lesions on the fins were observed in dead fish challenged by both methods. It is suspected that S. iniae entered through the body surface such as abrasive sites of the fins to cause disease.
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