Abstract
<p>Although several naturally available drugs have been historically used for the treatment of diabetes mellitus throughout the world, few of them have been validated by scientific criteria. Before approval of any drug developed it should pass through animal trial prior to clinical human trial, which should followed by some standard ethical rules. Recently, a large diversity of animal models have been developed to better understand the pathogenesis of diabetes mellitus, and new drugs have been introduced in the market to treat this autoimmune disease. In the present article, we demonstrated some standard handling procedure of animal trial for the approval of anti-diabetic drug, which could be helpful for both academics and industrial scientific community to conduct the animal experiments. This research also contributes in the field of ethnopharmacology to design new strategies for the development of novel drugs to treat this serious condition of diabetes mellitus that constitutes a global public health.</p><p> </p><p><strong>VIDEO CLIPS</strong></p><p><a href="https://www.youtube.com/v/_Qz4opKbNuc">Handling and caring of mice:</a> 2 min 30 sec</p><p><a href="https://www.youtube.com/v/1ftT8ozWy-c">Inducing diabetes in mice and observing blood glucose level:</a> 1 min 47 sec</p><p><a href="https://www.youtube.com/v/u01ls9p6310">Drug administration and observation of blood glucose level:</a> 2 min 11 sec</p><p> </p>
Highlights
The increasing worldwide incidence of diabetes mellitus in adults constitutes a global public health burden
There is a great interest in the development of new drugs to prevent the burden of complications associated with this disease and the raised interest in the scientific community to evaluate drug/ products in experimental studies in animal models and few of them were tested in humans (Frode and Medeiros, 2008)
In the light of the results, our study indicates that the tested drug has anti-diabetic activity as confirmed by the visual demonstration and differences in the blood glucose levels of control and treatment mice
Summary
The increasing worldwide incidence of diabetes mellitus in adults constitutes a global public health burden. Any newly developed anti-diabetic drug before its commercialization should be tested for its effect on diabetic animals. We demonstrated a safe method for producing diabetic condition in mice to be tested for anti-diabetic effect of any pharmaceutical drug. Streptozotocin (STZ, 69%) and alloxan (31%) are by far the most frequently used drugs, and this model has been useful for the study of multiple aspects of the disease. Both drugs exert their diabetogenic action when they are administered parenterally: intravenously, intraperitoneally or subcutaneously. Here in the present study, we modified and observed the dose pattern of streptozotocin for safe diabetes induction in experimental mice before drug administration.
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