Experimental research on the novel small-caliber vascular prosthesis basing on controlled release of heparin from mesoporous channel

Abstract

Objective To fabricate the mesoporous-based heparin-loaded expanded polytetrafluoroethylene (ePTFE) vascular prosthesis and evaluate their biocompatibility.Methods The study included the mesoporous-based vascular prosthesis group and the control group.The chromogenic assay.Pressing-perfusion test,protein adsorption assay and lactate dehydrogenase (LDH) cytotoxicity assay were determined to evaluate hemocompatibility and compatibility of vascular prosthesis.Results The heparin-released mesoporous-based vascular prosthesis weas synthesized successfully.The chromogenic assay confirmed that heparin-loaded keep lasting activity than the control vascular prosthesis group (P ＜ 0.05).HMVP-n keep compliance of original vascular prosthesis (P ＞ 0.05).The adsorption of fibrinogen on the surface of HMVP-n membranes is effectively reduced (OD values:0.29 ±0.12,0.31 ±0.06 and 0.30 ± 0.07,respectively),but HMVP-n grafts attracted higher amount of albumin (OD values:0.390 ± 0.150,0.327 ± 0.210 and 0.332 ± 0.240,respectively) compared than unmodified ePTFE graft (P ＜ 0.05),HMVP-1 especially.there was not significant difference of A values between HMVP-n and unmodified material (P ＞0.05) by LDH Cytotoxicity Assay.Conclusion The heparin-released mesoporous-based vascular prosthesis keep lasting antithrombotic activity and has good biocompatibility. Key words: Expanded polytetrafluoroethylene; Artificial vascular prosthesis ; Mesoporous ; Heparin; Biocompatibility