Abstract
Objective To explore the changes of type H vessel during the low bone mineral density caused by iron accu-mulation and discuss its clinical meaning. Methods Ten 8-week old male C57BL/6J mice were used for experiments, and ran-domly divided into two groups: control group and iron group, and 5 mice in each group. In the iron group, 0.1 g/kg of iron dextran was injected intraperitoneally once a week for 8 weeks. The control group was injected with the same amount of saline. The femoral and tibial specimens were examined by microscopic CT scan and bone tissue type H vessel immunohistochemical staining. Liver tissue from the two groups were collected for the content of iron by atomic absorption spectroscopy. All experimental data were ana-lyzed with t-test. Results The content of hepatic iron in mice was significantly higher than that in the control group, which indi-cating that the model was successfully established. The tibia specimens were collected for immunostaining. The vascular area of type H at metaphyseal regions is 11.24%±1.76% in iron group and 30.69%±2.78% in control group, respectively. There is signifi-cant difference between the two groups (P<0.005). The femur specimens were collected for Micro-CT scan, the value of bone miner-al density (BMD), bone volume/tissue volume (BV/TV), trabecular thickness (Tb. Th), trabecular number (Tb. N) and trabecular separation (Tb. Sp) was (0.19±0.013) g/cm3, 11.92%±1.199%, (35.66±2.684) μm, (2.36±0.429)/mm and (284.41±23.197) μm in iron group and (0.37±0.023) g/cm3, 35.76%±1.336%, (62.05±2.238) μm, (5.68±1.039)/mm and (163.23±13.203) μm in control group, respectively. The differences between the two groups were statistically significant (P<0.05). Conclusion Iron accumula-tion can lead to low BMD and suppress type H vessel formation in bone, which might provide a new experimental value for mecha-nism research on osteoporosis caused by iron accumulation. Key words: Animal experimentation; Bone density; Osteoporosis
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