Experimental research of subcutaneous scar formation caused by infusion leakage

Abstract

Objective To analyze the causes of subcutaneous scar formation caused by infusion leakage with different drugs. Methods A total of 32 SPF adult chickens were randomly divided into four groups: normal saline group (group A), clindamycin+normal saline group (group B), benzyl alcohol+normal saline group (group C), clindamycin+benzyl alcohol+ normal saline group (group D), with 8 chickens in each group. The above-mentioned drugs were injected into the chickens’ dorsal subcutis nearby the third metatarsal and metatarsophalangeal joint to establish the subcutaneous infusion leakage models. Double feet were injected at the same time, with 5 ml drugs for each side. All the chickens were executed 28 days after drug injection and pathological observation of subcutaneous tissue was performed. Then the harvested tissue of specimen was observed through the microscope after HE staining and Masson staining. Results The subcutaneous tissue of group A and B had no hyperplasia, while that of group C had hyperplasia but no scar formation. In group D, a large number of scar tissue was formed. Conclusion Benzyl alcohol may be the main cause of subcutaneous scar formation, and it may be synergistic with clindamycin to promote scar formation. Key words: Clindamycin; Benzyl alcohols; Infusion leakage; Scar formation