Experimental research of Haizao-Xiangsheng mixture in the treatment of chronic pharyngitis

Abstract

Objective To observe the effect of Haizao-Xiangsheng mixture on signs and symptoms, blood index and tissue pathology of rabbit models with chronic pharyngitis. Methods 60 Japanese white rabbits by random grouping method were divided into normal control group, model group, positive control group and Haizao- Xiangsheng mixture with high, medium and low dose group, with 10 rats in each group. Except the normal control group, rabbits in other groups were performed spraying ammonia in pharyngeal portion and submucosal injection of turpentine oil preparation to create chronic pharyngitis models. The Haizao-Xiangsheng mixture, high, medium and low dose group were treated with Haizao-Xiangsheng mixture of 42, 21, 10.5 g/kg, the positive control group was treated with watermelon frost Runhou tablet suspension 5.04 g/kg, the normal control group and model group were treated with the same volume of saline. Animal signs and symptoms were recorded daily (including general conditions and temperature). After continuous administration of 7 days, red blood cell count (RBC), white blood cell count (WBC), percentage of neutrophils (N%), the percentage of mononuclear cells (M%) and percentage of lymphocytes (L%) were detected to observe the histological change of pharyngeal mucosa and lower tissue pathology. Results After administration, compared with the model group, high dose group of Haizao-Xiangsheng mixture rabbit body temperature dropped (38.90 ± 0.21 ℃ vs. 39.23 ± 0.39 ℃, P< 0.05), WBC (10.1 ×1012/L ± 1.73 ×1012/L vs. 13.1 ×1012/L ± 1.69 ×1012/L) decreased, and RBC increased (5.73 ×109/L ± 0.59 ×109/L vs. 5.19 ×109/L ± 0.43 ×109/L, P<0.01). Conclusions Haizao-Xiangsheng mixture can improve signs and symptoms, hematology indexes and histopathological changes of the experimental rabbit models of chronic pharyngitis. Key words: Haizao-Xiangsheng mixture; Chronic pharyngitis; Signs and symptoms; Blood examination