Experimental reconstruction of mouse eggs and embryos: an analysis of mammalian development.

Abstract

The scope of experimental approaches applicable to the study of mammalian eggs and embryos has advanced in recent years to provide unprecedented opportunities for understanding mammalian embryology. Amongst these significant advances has been the ability to alter the genetic constitution of eggs by pronuclear and nuclear transplantation as well as by the introduction of specific cloned genes into eggs and embryos. These techniques can be used in conjunction with the experimental reconstruction of preimplantation embryos to investigate more precisely a number of aspects of mammalian embryology. Recently, a most intriguing aspect of development has been uncovered, one that is apparently unique to mammals; experiments have revealed that the parental genomes are not functionally equivalent during embryogenesis. Hence, the parental origin of chromosomes determines their influence during embryogenesis. The mechanistic aspects responsible for the germ line modifications of homologous chromosomes, their role during development, and the wide-ranging implications of these findings for mammalian development have yet to be fully defined. An understanding of this process will provide the basis for developing genetic and reproductive strategies that can be applied to domestic animals and to humans.