Abstract
Development of new effective treatments for glaucomatous optic neuropathy is one of the most acute aspects of modern ophthalmology. The aim of the work is to investigate the effectiveness of cell therapy with postnatal multipotent neural crest stem cells (NCSCs) using different cell delivery methods in a model of adrenaline-induced glaucoma. Materials and methods. Glaucoma was induced in Wistar rats by intraperitoneal injections of 10 μg to 15 μg/100 g body weight of 0.18 % adrenaline hydrotartrate. NCSCs were delivered intravenously (5 million cells), retrobulbarly (0.5 million cells) or parabulbarly (0.5 million cells). Histomorphometric analysis of the retina was performed on stained haematoxylin-eosin sections with a thickness of 5 μm one month after the delivery of NCSCs. Results. NCSCs transplantation by all modes of delivery caused positive morphological changes to varying degrees. Intravenous administration induced a decrease in edema in all retinal layers and a slight restoration of the cytoarchitectonics of the retinal layers. The parabulbar administration of NCSCs led to a decrease in edema and the restoration of the cytoarchitectonics of the layers, most pronouncedly the ganglion cell layer and the inner retinal layer. After the retrobulbar administration of NCSCs, the reduction in edema and restoration of the cytoarchitectonics of the layers were the most pronounced. Conclusions. According to the results of the study, the positive effect of NCSCs transplantation in an experimental model of glaucoma was the most pronounced following the retrobulbar injection of cells. Further investigations of the mechanisms of the effect of transplanted NCSCs on retinal structure restoration are needed.
Highlights
Glaucoma is one of the leading causes of blindness and disability worldwide [1, 2]
We focused on multipotent neural crest stem cells (NCSCs)
In this experiment, a positive effect of NCSCs transplantation was observed in an adrenaline-induced glaucoma model
Summary
According to an epidemiological study, the number of people with glaucoma will increase by 74 % from 2013 to 2040 [3]. Glaucoma is defined as progressive optic neuropathy characterized by the excitation of the optic disc and a corresponding decrease in retinal sensitivity [1, 4]. Glaucoma optic neuropathy (2020), «EUREKA: Health Sciences» Number 2 involves the progressive loss of retinal ganglion cells and their axons, the induction of which is influenced by both external and internal factors [5, 6]. Many experimental studies using modern science techniques have been carried out, and it has been shown that multipotent stem cells have a positive effect in ophthalmology [7]. Advances in regenerative medicine and cell therapy are based on the unique properties of stem cells, including their ability to self-renew and differentiate into specific cell types, and the prospects for clinical use are virtually limitless [8,9,10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
