Abstract
Probably the most serious types of peritonitis are those in which not only virulent micro-organisms are present in the peritoneal cavity, but, in addition, culture material for the micro-organisms to develop on. This set of conditions is seen in various types of perforative peritonitis or in lesions accompanied by the death of tissue, such as postoperative peritonitis or peritonitis with strangulated hernia. Since it is difficult to produce peritonitis experimentally by the introduction of bacteria alone into the normal peritoneum, it seemed logical to assume that experimental peritonitis could be produced by the introduction of bacteria growing on a culture medium. By such a method it would be possible, theoretically, to study peritonitis produced by a single type of micro-organism or by selected groups of micro-organisms. Accordingly, pure cultures of Bacillus coli, staphylococcus, streptococcus and pneumococcus on agar or blood agar slants were introduced into the peritoneal cavity of dogs
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