Abstract
[Objective] To observe the retransmission of radiation side effects between cells. [Materials and Methods] The mouse ovarian cancer cell line NUTU19 was irradiated with 6MV-X-rays, and the culture medium was prepared for the first-generation conditioned medium. The first-generation effector cells were used to detect the NUTU19 cell line and the intestinal mucosal epithelial IEC-6 cell line. The secretion of effector cells was a second-generation conditioned medium, and the second-generation effector cells NUTU19, IEC-6, and mouse lymphocytes were treated to measure cell viability and apoptosis. [Results] After treated with NUTU19 second-generation medium for 48 hours, the apoptosis rate of IEC-6 and NUTU19 cells was promoted (p>0.05), and the apoptosis rate of lymphocytes was decreased (p 0.05). After treatment with IEC-6 second-generation medium for 48h, the apoptosis of NUTU19 and IEC-6 was promoted (p 0.05). It promoted the apoptosis of NUTU19 (p<0.05). [Conclusion]: Under certain conditions, tumor cells, intestinal epithelial cells and lymphocytes can retransmit the damage of the side effect of radiation.
Highlights
Since the first observation of the radiation side effect in 1992 [1], it has been found that the side effect in vivo is a "double-edged sword"
NUTU19 Tumor cell lines (NUTU19), normal epithelial cells (IEC-6), and lymphocytes treated with the primary culture solution were cultured for 24 h and 48 h, respectively, and parallel controls were set
Compared with the control group, proliferation of NUTU19 cells and IEC-6 cells was inhibited, and there was no significant effect on lymphocyte proliferation activity
Summary
Since the first observation of the radiation side effect in 1992 [1], it has been found that the side effect in vivo is a "double-edged sword". In patients with kidney cancer and breast cancer who are treated with local radiation, it is found that unaffected tumor tissue has lesional damage [2,3]. Experimental and clinical observations of more normal tissue damage in non-irradiated areas [5,6,7,8,9,10] can be over long distances and extra long time. When formulating the physical plan of radiotherapy, the side effect damage did not receive enough attention, and there was not enough theoretical support for its mechanism and how to antagonize it. Will this injury become one of the limiting factors in clinical radiotherapy?
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