Experimental Myocarditis Produced by Myoglobin

Abstract

The purpose of this study is to find the relationship between myoglobin (Mb) and myocarditis. The experiment on the following various conditions was undergone : (1) the mixture of Mb with phosphatide (muscle). (2) the mixture of streptococcus hemolyticus and streptococcus viridans. (3) the combined steroidhormon plus NaH2PO4 and addition of heavy exercise. In the above condition the study was done to clarify the significance of Mb. Method The animals used are rabbits with an average initial body weight of 2 kg. The experiment was divided into following three parts. First experiment : The Mb used was extracted from the skeletal or heart muscle and diaphragm in rice-illness rabbits. The phosphatide extracted from skeletal muscle in rice-illness rabbits. The rabbits were sensitized by intravenous innoculations of the mixture with above mentioned Mb and phosphatide in one group of rabbits was injected for twenty days, the other group in the first phase was injected for five days and after two weeks treated again for five days. Second experiment : In the second experiment the rabbits were sensitized by intravenous innoculations of Mb which was extracted from normal rabbits, combined with streptococcus hemolyticus, streptococcus viridans and bovine serum. Third experiment : The normal rabbits were sensitized by intravenous innoculations of Mb of focal lesion (Oleum terebintinhae abscess). Furthermore the rabbits were treated with steroidhormon plus NaH2PO4 Mb or stressors (restraint or heavy exercise). Hydrocortisone and Predni-solone were used as the steroidhormon. After receiving treatment, the animals were bled to death. The histologic examination was made on the heart, lung, liver, spleen and adrenal gland. These organs were fixed with ten per cent formalin solution, embedded in paraffin and stained with hematoxylin and eosin.