EXPERIMENTAL MODELING OF MULTIPLE SCLEROSIS: THE RESULTS OF CORRECTIVE ACTION OF ACTIVE ANTIOXIDANT

Abstract

Comprehensive treatment of patients with multiple sclerosis (MS) is still relevant. The complex pathogenesis and acute incidence of MS in young people requires the use of multifunctional drugs with a protective effect, including antioxidants. The purpose of this study is to identify a corrective neuroprotective and immune modulating effect of the synthetic drug Triafen-2 (Trf-2), obtained in optimized way. Trf-2 was used at a dose of 50 mg/kg orally with chronic administration in II parts of experiments. To create an adequate model of MS - autoimmune encephalomyelitis (EAE), immunization of guinea pigs with an encephalitic mixture was used in Freund’s complete adjuvant. Mortality, a number of immunological parameters, the clinical picture of neurological complications and the severity of CNS damage in guinea pigs were determined. We studied morphologically the development of T-lymphocytic infiltration of the brain and spinal cord, demyelination of nerve fibers, the degree of cerebellar disorders, disorders of the pelvic organs, the presence of paresis and paralysis. A significant decrease in mortality was shown and protective effect of Trf-2 on neurological symptoms of EAE was noted. The reduction and even complete absence of paresis and paralysis in 66.5% of guinea pigs in the experimental group compared with animals that did not receive treatment was determined. A positive effect of Trf-2 on the migration activity of leukocytes and antibodies in the blood was found. In the group with Trf-2, there was a decrease in the content of lipid hydroperoxides and malonic dialdehyde. A pronounced neuroprotective and immune modulating effect of Triafen-2, which inhibits pathological disorders in an experimental model of EAE, has been established. The results justify the need for further study of Triafen-2 as a promising corrective drug.