Abstract
Introduction: The consumption of fructose has been questioned, since its increase has led to an associated increase in steatosis caused by nonalcoholic fatty liver disease. Despite the advantages presented by the zebrafish as an animal model, at present there are no models of steatosis by fructose in adult zebrafish. The aim of this study is to establish a model of hepatic steatosis by fructose in adult zebrafish. Methods: Firstly, adult zebrafish were daily exposed to 4% or 6% fructose. Then, animals were exposed to 6% fructose every 2 days. The hepatic lipid accumulation was analyzed by Nile Red and Oil Red O staining. Results: The daily exposure to 6% fructose showed increased accumulation of hepatic lipids when compared to 4% and control groups, but the same concentration showed no difference when the exposure happened every 2 days. Conclusion: We can suggest the daily exposure to a concentration of 6% fructose can be considered as a new experimental model of adult zebrafish. Keywords: Fatty liver; fructose; zebrafish
Highlights
ObjectivesThe aim of this study is to establish a model of hepatic steatosis by fructose in adult zebrafish
The consumption of fructose has been questioned, since its increase has led to an associated increase in steatosis caused by nonalcoholic fatty liver disease
We can suggest the daily exposure to a concentration of 6% fructose can be considered as a new experimental model of adult zebrafish
Summary
The aim of this study is to establish a model of hepatic steatosis by fructose in adult zebrafish. Since the objective of this study was first to determine the amount of fructose (4% or 6%) as well as the type of induction necessary for generation of hepatic steatosis, we hardly performed histological analyzes to use a minimum number of animals
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