Abstract
To describe an effective experimental model to study the Achilles tendon healing. Forty male Rattus norvegicus albinus, Wistar lineage adult male weighing 250 to 300g were used for this experiment and thirty were surgically submitted to bilateral partial transverse section of the Achilles tendon. The right tendon was treated with radio waves (RF) whereas the left tendon served as control. On the third postoperative day, the rats were divided into four experimental groups consisting of ten rats each which were treated with monopolar RF adjusted to 650 kHz and 2w, for two minutes twice a week and a group of normal animals without any intervention, until they were sacrificed on the 7th, 14th and 28th days, respectively. Tendons were weighed and collagen quantification was evaluated by hydroxyprolin content. Significant reduction in collagen content on day 7, 14 and 28 was related to control experiment to normal tendon (7 days, p<0.01; 14 e 28 days, p<0.05). The experimental model has been effective and available to be used to study Achilles tendon healing.
Highlights
MethodsInjuries to tendons are among the most common injuries to the body
In order to determine if a proposed animal tendon injury model is appropriate a number of conditions must be met
We describe a protocol for Achilles tendon injury in rats showing its effectiveness by quantifying 4-hydroxyprolina on phases of tendon regeneration and using the contralateral paw as control
Summary
MethodsInjuries to tendons are among the most common injuries to the body. They include complete tendon ruptures that occur from a single overload event as well as the less dramatic but more common incomplete injuries such as tendinitis. VIIIPhD, Associate Professor, Division of Human Structural Topography, Department of Surgery, Medical Research Laboratory, FMUSP, Sao Paulo-SP Brazil. ABSTRACT PURPOSE: To describe an effective experimental model to study the Achilles tendon healing.
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