Abstract
ABSTRACTFor gene transfer in laboratory-cultivated mouse embryonic stem cells (mESCs), previously designed recombinant gene constructs with respective genes inserted in them are necessary. For this aim, recombinant DNA-genomes from adeno-associated virus (AAV) (Parvoviridae), containing promoter of gene, coding Elongation Factor 1-alpha (EF1-α); isolated from 3T3 fibroblasts of adult laboratory mice Balb/c inserted oncogene Dcn1, (in its role of regulator on the tumor-suppressor gene p53 by specific pathways of indirect inhibition), as well as gene for neomycin resistance, isolated from bacterial DNA-plasmid, are used for gene transfection by electroporation. On the other hand, eventual subsequent super-activation of tumor-suppressor genes both in vitro and in vivo is also necessary.
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