Abstract
LEPTOSPIRAL agglutinins or agglutinating factors have been reported in poikilothermic vertebrates collected from the natural environment1–5. Pathogenic leptospires have been isolated from6,7 and experimentally propagated1,8 in lower vertebrates. During follow-up of a human leptospirosis outbreak associated with swimming9,10, leptospires (unidentified) were isolated from the kidney tissue of frogs collected at the place of swimming11. Frogs were inoculated with the leptospiral isolate in an attempt to: (a) produce infection and disease; (b) measure an antibody response; (c) propagate the organism; and (d) develop carriers and shedders.
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