Experimental investigations of diel vertical movements by lotic mayflies over substrate surfaces

Abstract

SUMMARY. 1. The vertical distribution within the substrate of three lotic mayflies (Rhithrogena sp., Paraleptophlebia sp. and Baetis tricaudalus Dodds) was examined in laboratory streams to determine if diel patterns of vertical movement across substrate surfaces existed and whether light intensity, temperature, substrate composition or food availability influenced the observed movement patterns.2. Rhithrogena sp. and B. tricaudatus exhibited significant diel movement from lower substrate surfaces during the day to upper surfaces at night, although >64% of B. tricaudalus occupied the upper surfaces throughout the 24 h period. In contrast, Parakplophlebia sp. larvae did not change their vertical distribution over the 24 h period.3. The proportion of mayflies occupying substrate surfaces was influenced by the amount of upper and lower surface area available in the substrate and, possibly, crevice sizes associated with lower surfaces.4. The movement of Rhiihrogena sp. from lower surfaces during the day to the upper surfaces during the night was influenced by changes in light intensity, but not by changes in food availability or water temperature.5. The diel patterns of vertical movement exhibited by lotic mayflies in this investigation raise the possibility that at least some biotic interactions may vary over a diel cycle. These could include competitive interactions or temporal cycling of prey availability to predators.