Abstract
We have investigated experimentally the pattern formation of bacterial colonies. We have especially examined DBM (dense-branching-morphology)-like colonies of bacterial species Bacillus subtilis . It was found from microscopic observations that many active bacterial cells collect and make a group on the tip of each growing branch. Branches repel each other and split sometimes as they grow outward. It was confirmed macroscopically that both averaged branch width and averaged branch gap decrease systematically when increasing the nutrient concentration C n , while their ratio remains unchanged with the approximate value of one. It was also found that the distribution of branch lengths is very close to exponential, suggesting that the tip-splitting of branches takes place at random.
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