Abstract
The main aim of this paper is to achieve the suitable SA-GEL (sodium alginate and gelatin) porous cartilage scaffold by 3D printing technology with optimal prediction parameters. Firstly, the characteristics of SA-GEL were analyzed, the influence of calcium chloride on the gel was explored, and the optimal cross-linking concentration and gelation temperature were determined. Secondly, a prediction model of the extrusion line width of SA-GEL was established, in which the printing pressure, the moving speed of the needle and the fiber interval were the important parameters affecting the printing performance of the SA-GEL composite material. Thirdly, the SA-GEL composite scaffolds were printed on the Bio-plotter platform, the C5.18 chondrocytes cells were cultured in the SA-GEL biomaterial scaffold, and the results show that the cells could survive well. These results show that, under the control of the printing parameters pressure 1.8 bar, moving speed 10.7 mm/s and the internal structure parameters of the scaffold is 0/45-1.2 (Printing interval: 1.2 mm, angle value: 45 degree), SA-GEL scaffold printing results can be obtained which have good mechanical properties and biocompatibility.
Highlights
Articular cartilage injury is a common and frequently occurring disease
Homogeneoussodium sodiumalginate alginate solution
Distilled water, and each solution was mixed with a gelatin solution of various concentrations, The homogeneous solution of sodium alginate and gelatin (SA-GEL)
Summary
Articular cartilage injury is a common and frequently occurring disease. Due to the lack of nutrition channels for mature joints, its regeneration ability is limited, and it is difficult to recover to its original state in case of injury [1]. Transparent cartilage scaffold has been constructed by cartilage tissue engineering in vitro and in vivo, which provides a new method for repairing cartilage defects [4,5,6]. Cartilage tissue engineering takes chondrocytes extracted from the patient’s cartilage as seed cells, which are cultured and proliferated in vitro, and inoculates them into biodegradable scaffolds with good mechanical properties and biocompatibility. The composite of cell scaffolds is implanted into the human body, and the chondrocytes on the scaffolds are proliferated
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