Abstract
For many years, pseudorabies has been efficiently controlled in China by vaccination. However, a sudden outbreak occurred on many pig farms during 2012-2013. In this study, phylogenetic analysis was carried out with the aim of understanding the genetic relatedness between strains in both populations and comparing strains in China with those circulating in other countries. To obtain more pathogenetic relevant data regarding two pseudorabies virus (PRV) isolates (SH2010 and SH2012) in sheep, we evaluated samples of PRV isolated in Shanghai, China between 2010 and 2012. Based on clinical signs, body temperature variation, and the maximum amounts of viral DNA in unvaccinated/infected sheep, the emerging PRV variant strain, SH2012 was observed to be more highly virulent than the traditional PRV SH2010 strain. The data of the present study will aid in a better understanding of the differences in pathogenicity between the traditional PRV SH2010 strain and the emerging PRV variant strain SH2012.
Highlights
Pseudorabies virus (PRV), which belongs to the family Herpesviridae, subfamily Alphaherpesvirinae, and genus Varicellovirus, is the causative agent of Aujeszky’s disease [1]
To obtain more pathogenetic relevant data regarding the two PRV isolates (SH2010 and SH2012) in sheep, we evaluated PRV samples isolated in Shanghai, China between 2010 and 2012
After continuous observation for 14 days, sheep in group A were inoculated intramuscularly with 1,000 50% lethal doses (LD50) of the PRV SH2010 strain, and sheep in group B were challenged with 1,000 LD50 of the SH2012 strain
Summary
Pseudorabies virus (PRV), which belongs to the family Herpesviridae, subfamily Alphaherpesvirinae, and genus Varicellovirus, is the causative agent of Aujeszky’s disease [1]. PRV strain SH2010 was isolated from unvaccinated pigs on an infected farm in Shanghai, China, in 2010. PRV strain SH2012 was isolated in May 2012 from neonatal piglets showing severe neurological signs, including tremors, convulsions, and opisthotonus, during a PR outbreak on a pig farm that rou-tinely used the commercial Bartha-K61 vaccine.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
