Abstract
1. 1. One week after treatment with a single dose of estradiol, the vitellogenin (Vg) concentration was significantly higher in the freshwater group than in the sea-water group. 2. 2. DNA concentration in the liver was increased in the freshwater group compared to the control, while there was no effect on the sea-water group. 3. 3. RNA concentration in the liver was significantly increased in both groups compared to the controls. 4. 4. Treatment with estradiol did not affect the osmotic concentration when compared to the control group, but a significant decrease is seen in the freshwater control group compared to the sea-water control group. 5. 5. Treatment with estradiol decreased the concentration of chloride in plasma in both sea-water and freshwater groups compared to the controls. 6. 6. Gel filtration of plasma from estradiol-treated animals shows that the elution profile contains a peak, which coincides with a Vg peak. 7. 7. One week after treatment with estradiol the concentration of estradiol in plasma in the treated sea-water group was twice as high as in the treated freshwater group.
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