Abstract
A method for producing semi-quantitative infections of the filarial worm, Litomosoides carinii, is described. Tropical rat mites, Bdellonyssus bacoti, were fed on infected cotton rats, Sigmodon hispidus texianus. Infective larvae were dissected from the mites in half strength Tyrode's solution and transferred in counted numbers to subcutaneous pockets formed by surgical incision in the skin of cotton rats. Intact mites and mites in whose integument a slit had been cut were transferred to subcutaneous pockets, the abdominal cavity and the stomach. Larvae were also transferred to the mouths of cotton rats. The results indicate that larvae cannot escape from intact mites except when these are feeding, and that infection does not occur by way of the mouth or stomach. Larvae migrate from the skin to the pleural cavity within a few days and before they undergo appreciable growth. Most larvae migrate equally promptly from the abdominal cavity although a few remain there and develop normally.
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