Abstract
ABSTRACTCarbohydrates are an important component of soil organic matter, and a method is needed to quantify them, which would be efficient in terms of time and cost. Different extractants and methods were examined in this work for their efficiency to extract carbohydrate C from four calcareous soils. Four extractants (distilled water, 0.5 M potassium sulfate (K2SO4), and 0.25 and 0.5 M sulfuric acid (H2SO4)) and three incubation methods (shaking for 16 h, heating in an oven (85 °C) for 16 h, and heating in a water bath (85 °C) for 2.5 h) were compared. The results show that significantly more carbohydrate C was extracted from all four soils with oven and water bath heating of the soil–extractant suspensions than with shaking them at room temperature. The efficiency of the extractants decreased in this order: 0.5 M H2SO4 > 0.25 M H2SO4 > 0.5 M K2SO4. The combination of the heated–water bath incubation method with 0.5 M H2SO4 as extractant was the most efficient method.
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