Abstract
Virus particle concentration is a critical piece of information for virology, viral vaccines and gene therapy research. We tested a novel nanoparticle counting device, “Videodrop”, for its efficacy in titering and characterization of virus particles. The Videodrop nanoparticle counter is based on interferometric light microscopy (ILM). The method allows the detection of particles under the diffraction limit capabilities of conventional light microscopy. We analyzed lenti-, adeno-, and baculovirus samples in different concentrations and compared the readings against traditional titering and characterization methods. The tested Videodrop particle counter is especially useful when measuring high-concentration purified virus preparations. Certain non-purified sample types or small viruses may be impossible to characterize or may require the use of standard curve or background subtraction methods, which increases the duration of the analysis. Together, our testing shows that Videodrop is a reasonable option for virus particle counting in situations where a moderate number of samples need to be analyzed quickly.
Highlights
Virus particle concentration is critical for most research involving viruses, including virus-based gene therapy vectors, vaccines or virus-like particles
In 2016, Boccara et al [17] developed a simple device based on interferometric light microscopy (ILM) for fast virus counting from environmental sources [18]
Cell culture media led to particle counts with a raw value and blank-corrected mean of 8.22 × 108 (CV% 45.7) and 2.67 × 107 (CV% 115) particles per milliliter (pp/mL), respectively
Summary
Virus particle concentration is critical for most research involving viruses, including virus-based gene therapy vectors, vaccines or virus-like particles. In 2016, Boccara et al [17] developed a simple device based on interferometric light microscopy (ILM) for fast virus counting from environmental sources [18]. This technology was further developed and commercialized by the Myriade Videodrop is based on a transmission brightfield microscope used as a homodyne interferometer to detect, count and track nanoparticles (NPs). Counting particles from a defined volume directly leads to the concentration, while tracking the Brownian motion allows the measurement of their
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