Experimental Esophagitis Model Preventing Tracheal Aspiration.

Abstract

Corrosive esophagitis injuries are aserious clinical problem with many agents used for diagnosis and treatment. Experimental esophagus burn models use amethod described mainly in studies by Gehanno and Guedon, and modified by Liu and Richardson. The aim of this study was to describe anew esophagitis model that prevents tracheal aspiration. In this study we used 16 Wistar albino rats weighing between 220-240g. The experimental animals were randomly divided into two groups: the model group (group M, n: 8) and control group (group C, n:8). The necessary anesthesia was administered. Passing through amedian laparotomy incision, the abdomen was entered and in group Mand Cthe esophagus was freed and held by asuture at the gastroesophageal junction. After this procedure, about 1cm proximal to the gastroesophageal junction, the esophagus was suspended by asuture. The esophagus segment between the two sutures was exposed to 0.1mL 10% NaOH in group Mand 0.1mL saline in group Cfor 20s. Ten days later all experimental animals were sacrificed and their esophagus removed. After dying with hematoxylin and eosine trichrome, the histopathological evaluation results for the rats in all groups were investigated with alight microscope. Histopathological examination indicated submucosal collagen increase, damage to muscularis mucosa and tunica muscularis and collagen deposition. In the model group, the rats had high neutrophils and tissue damage accompanied by necrosis. In the control group, the rats had minimal or no tissue damage and fibrosis was not observed. Our procedure is relatively less invasive and easy to apply with corrosive esophagitis only in the required region, and at the same time treatment medications can be easily administered.