Experimental Design For 1,3-Propanediol Biosynthesis by K. Pneumoniae GLC29 Using Glycerol

Abstract

Biodiesel glycerol can be used for the production of 1,3-propanediol by bacteria. In this study on the production of 1,3-propanediol by Klebsiella pneumoniae GLC29 was possible to optimize media culture the production of 1,3-propanediol using statistical experimental design techniques and response surface methodology, where 11 variables were tested and only 5 were found significant (glycerol, yeast extract, ammonium sulfate, vitamin B12 and fumarate). Subsequently, production of 1,3-propanediol was verified in 0,75 liter reactors, both in batch and exponential fed-batch. It was possible to achieve 23.6g/l of 1,3-propanediol in batch cultures using pure glycerol, 27g/l of 1,3-propanediol in batch cultures using biodiesel glycerol, and 29.9g/l of 1,3-propanediol in exponential fed-batch using biodiesel glycerol.