Experimental characterization of the elution profiles of high concentration chromatographic bands using the analytical solution of the ideal model

Abstract

Experimental results are compared to the predictions derived from the exact solution of the ideal model of chromatography. Although the efficiency of the column used was around 5000 theoretical plates, there is a close agreement between the band profiles recorded for large samples injected on different chromatographic systems and those predicted by the ideal model. The variations of the apparent column capacity factor and apparent column efficiency are also in excellent agreement with the theoretical results. This work confirms earlier findings, that the band profiles can be scaled when the adsorption isotherm of a compound in a chromatographic system follows the classical Langmuir behavior. The authors results can be used for a rapid determination of adsorption isotherms and an accurate prediction of the band profiles at various column loadings. They also permit the prediction of the dependence of the retention time and apparent column efficiency on the sample size.