Experimental assessment of the relationship between the indigenous composition of the intestinal microflora and the elemental status of the common carp (Cyprinus caprio) against the background of the use of new feed additive

Abstract

Relevance. Aquaculture is an intensively developing industry worldwide and is an important source of food for the population. Satisfaction of nutritional needs in micronutrients is a physiologically significant part of fish nutrition and, as a result, ensures their health. Currently, various approaches are being used to ensure the resistance of fish to diseases of various etiologies and to increase their productivity. One of the promising methodological approaches is the study of the fish microbiome as a sustainable alternative to improve aquaculture methods.The aim of the study was to study the degree of relationship between the composition of the intestinal microflora and the level of essential elements in the body of carp (Cyprinus caprio) against the background of the use of feed additives “Bubitan” and “Intebio”.Methods. The object of research was carp yearlings (n = 60) grown in the conditions of LLC “Irikla-fish” (Orenburg region). Feed additives “Intebio” and “Butitan” were used as growth and development regulating factors. Dynamic indicators of changes in the species composition of the intestinal microbiome and elemental status were carried out using metagenomic sequencing, atomic emission (NPP-ISP) and mass spectrometry (MS-ISP) methods.Results.The data obtained indicate a significant effect of the tested phytobiotics on the indices of indigenous microflora. The closest indicators of the distribution of taxonomic groups of intestinal microbiocenosis in relation to the control were registered against the background of the use of the phytobiotic «Butitan». Correlation analysis of the data allows us to state with a high level of reliability that a significant increase in the number of microorganisms of the genus Hydrotalea and Flavobacterium has a positive effect on the degree of assimilation of macro- and microelements from the feed.