Abstract
The organism is a Gram positive, nonmotile bacillus three-tenths to six-tenths of a micron in diameter and one to three microns in length. The cells are straight or slightly curved and often club-shaped. Alternate bands of stained and unstained material appear when the cells are treated with Gram or methylene blue stains. Metachromatic granules may be demonstrated by Neisser's stain. Angular and palisade arrangement of the cells is frequent. On blood agar plates the organism produces round, convex colonies about one mm in diameter after 48 hours of incubation at 37 C. There is a slight greening of the medium. The colonies are white, opaque, and glistening with an even margin and smooth surface. Growth on unenriched nutrient agar is less abundant. In nutrient broth a granular turbidity and heavy sediment appear. No pellicle is formed. Gelatine is not liquefied. There is no visible growth on potato. On Loeffler slants there is moderate colorless growth, no peptonization. There is no change in litmus milk, and indol is not formed from tryptophane. Nitrite is produced from nitrate. The organism is aerobic. It grows anaerobically in the presence, but not in
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