Experimental approbation of polymer coatings of textile vascular prosthesis with prolonged antibacterial activity

Abstract

Objective. To study the resistance to medication washout from textile woven vascular prostheses with various types of polymer coatings experimentally.Materials and methods. Samples of a textile woven vascular prosthesis completely covered with one of three types of hydrogel coatings were examined. The resistance to washout of 1 % vancomycin and 0.2 % linezolid from the coatings was assessed by the magnitude of the antibacterial activity of the samples. The research methods were: disc diffusion and according to the ability to suppress the visible growth of Staphylococcus aureus ATCC 25923 (S. аureus) in Muller- Hinton broth.Results. All the samples of the textile woven vascular prosthesis with the coatings soaked for 30 minutes in 1 % vancomycin or 0.2 % linezolid solutions inhibit the growth of S. аureus culture on the surface of the agar, which leads to the formation of optically transparent sterility zones at the polymer-medium interface. The size of the sterility zone without washout is larger for all linezolid coatings. However, in 7 day washout, the size of the sterility zones is larger for all vancomycin coatings.Conclusion. Textile woven vascular prostheses with various types of polymer coatings and antibiotic vancomycin (1 %) are statistically significantly more resistant to washout during 7 days than with linezolid (0.2 %). The presence of L-aspartic acid in polymer coatings statistically significantly increases the resistance to antibiotic washout compared to hyaluronic acid, and the presence of polyvinylpyrrolidone statistically significantly reduces the resistance to antibiotic washout from polymer coatings.