Abstract
The modulation of antimicrobial properties of nanomaterials can be achieved through various physical and chemical processes, which ultimately affect subsequent properties. In this study, the antibacterial potential of nano-silver was investigated at 0.5, 1.0, 2.0, and 3.0g/L, and its differential temperature synthesis was achieved at 20, 50, and 70°C using the solvent evaporation method. Nano-silver particles exhibited FCC (octahedral) crystalline structure with crystallite sizes ranging between 28 and 39nm calculated using XRD analysis. Moreover, irregular and non-uniform surface morphology was evident from SEM micrographs. The UV-Vis absorbance spectrum of nano-silver exhibited wave maxima at 433nm, while the FTIR analysis depicted different modes of vibration indicating the CH, OH, C≡C, C-Cl, and CH2 functional groups attached to the surface. Lastly, nano-silver caused prominent inhibition (12.5mm) in the Escherichia coli growth, particularly at 70°C synthesis temperature and 3.0g/L dose. It is concluded that both the nano-silver crystal growth temperature and dose contributed substantially to bacterial growth inhibition linked with subsequent size, shape-dependent properties.
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