Abstract
Mini-hydrocyclones have been increasingly used as retention devices to separate mammalian cells in the continuous cell culture. However, the cell viability loss after running through mini-hydrocyclones varies even for the same cell, e.g., the most widely used Chinese hamster ovary (CHO) cells. The reasons behind this problem are unclear. This paper presents a quantitative analysis of the CHO cell viability loss in mini-hydrocyclones with respect to the magnitude of shear stress. The viability loss of CHO cells is measured experimentally. The distributions of shear stress magnitude are revealed using a validated numerical model. The experimental results show that the cell viability loss increases with the increase of hydrocyclone cone angle or the inlet velocity. This result can be explained by the increased shear stress magnitude in the mini-hydrocyclones.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
