Abstract

Experimental and natural infections of Simulium sanchezi by Mansonella ozzardi were studied in the area of Siquita, Territorio Federal Amazonas, Venezuela. The microfilariae developed synchronously in the blackflies, reaching stage L3 in seven to eight days at temperatures between 23 ° and 27 °C. Larvae in different stages of development, including infective forms, were found in 0·6% of 662 unfed wild-caught females. These results confirm that simuliids are the main vectors of M. ozzardi in the American continent.

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