Abstract
The solubility of β-carotene in pure and ethanol-modified subcritical water was measured for the first time. Response surface methodology (BBD) was used to evaluate the experimental conditions. The independent variables were temperature (70–130) °C, subcritical water flow rate (0.2–0.64) mL/min and (0–10) % (v/v) of ethanol as co-solvent. Samples were analyzed by HPLC. The solubility of β-carotene was found as 272.341ppm in the optimum experimental conditions. Additionally, CPA EOS was used for describing the solubility of β-carotene in subcritical water and in ethanol. The binary regressed parameters were applied to predict the solubility of β-carotene in (ethanol+water) solution.
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