Abstract

NO consumption by RBCs in blood vessels is reported to be several folds lesser than free Hb. Mechanisms of reduced NO uptake by RBCs are under intense investigation. NO delivery to the RBCs is critical in quantification of interactions. Common methods of NO delivery include saturated NO solutions and NO‐donors that create localized NO concentration and have unsteady NO release rates, respectively. We designed a micron scale flow system in which precise amounts of gaseous NO can be delivered over longer periods through polymeric semi‐permeable membrane. The flow system simulates in vivo conditions like the constant NO flux from endothelium to the lumen, diffusional resistances for NO transport in the lumen and NO reaction with flowing RBCs. Using the flow system, we studied the effect of NO concentration, RBC flow rates and hematocrit on NO‐RBC interactions. NO‐RBC reaction products (nitrite and total nitrogen species (NOx)) were measured using chemiluminescence analyzer. Nitrite and total NOx formation increased with NO concentrations, however, nitrite and total NOx formations nearly doubled when RBC flow rate was halved. Nitrite and total NOx formations decreased with hematocrit. Results obtained from this study further enhance our understanding of the effects of hemodynamic parameters on the NO‐RBC interactions. Supported by: AHA 0530050N and NIH R01 HL084337 & R15 HL087287.

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