Abstract
Humic-like fluorescent dissolved organic matter (FDOM) have been widely used as tracers for bio-refractory dissolved organic matter to understand its dynamics in the oceans. Vertical distributions of humic-like FDOM are controlled by microbial production in the ocean interiors and photobleaching in surface layers. Although humic-like FDOM is expected to be actively produced in surface layers with high bacterial activity, its production in surface seawater is not well understood. To examine the diurnal variations in humic-like FDOM due to microbial production and photobleaching in surface seawater, we conducted seven experiments from night to day using surface seawater in the subtropical Pacific and coastal regions. Parallel factor analysis determined that FDOM in the incubated seawater was composed of three components: two types of humic-like FDOM and a protein-like FDOM. The fluorescence intensity of humic-like FDOM increased to 104.0 ± 2.5% of the initial intensity during the night and decreased to 101.2 ± 2.5% under sunlight exposure during the day. Conversely, its intensity significantly increased to 114.0 ± 2.7% under dark conditions during the day. The turnover rates of humic-like FDOM by the increase and decrease in its intensity were estimated to be 0.14 and 0.11 d–1, respectively. These comparable turnover rates indicated that the production and photobleaching of humic-like FDOM were almost in equilibrium in the surface layer, with a low level of humic-like FDOM. Linear correlations between the intensity of humic-like FDOM and concentrations of dissolved oxygen in all experiments under dark conditions indicated that humic-like FDOM were produced as the by-products of microbial respiration processes in the surface seawater. Using global bacterial respiration rates, the net production rate of humic-like FDOM in the global photic layer was estimated as 4.2–5.5 x 1017 R.U. yr–1, contributing to 75% of its production in the entire ocean.
Highlights
Marine dissolved organic matter (DOM) constitutes one of the largest pools of reduced carbon on the Earth’s surface, containing 662 Pg C, similar to atmospheric CO2 (Hansell et al, 2009)
C2 was characterized as marine humic-like fluorescent DOM (FDOM) (Coble, 1996), which was blue-shifted from Component 1 (C1) and exhibited two Ex maxima (
The diurnal variations in the fluorescence intensities of humic-like and tyrosine-like FDOM were demonstrated in incubation experiments using surface seawater
Summary
Marine dissolved organic matter (DOM) constitutes one of the largest pools of reduced carbon on the Earth’s surface, containing 662 Pg C, similar to atmospheric CO2 (Hansell et al, 2009). More than 90% of DOM is resistant to microbial degradation and has been estimated to remain in the ocean for many years, which is referred to as RDOM (Bauer et al, 1992; Hansell, 2013). The labile fraction of DOM is rapidly decomposed by bacteria, where semi-labile DOM and RDOM are produced as the by-products of bacterial metabolism (Benner and Herndl, 2011). The quantitative and qualitative changes taking place in the DOM in the surface layer due to microbial activity and photochemical reaction are difficult to understand because DOM is a mixture of many unidentified organic compounds (Dittmar and Paeng, 2009)
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