Abstract

In a previous paper the authors gave a detailed description of the process for the production of capillary ultrafiltration bioactive membranes. Whole cells of Sulfolobus solfataricus were entrapped within the polymeric matrix of asymmetric polysulphone membranes. In this paper the kinetic behaviour of a cross-flow bioreactor equipped with such membranes is discussed with reference to hydrolysis of lactose to glucose and galactose, catalyzed by a cytoplasmic β-galactosidase. An experimental investigation is presented of the effects of transmembrane pressure and substrate feed concentration on reactor performance, based on substrate conversion in the permeate. Long-term operation and storage indicate a remarkable kinetic stability of the immobilized cells.

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