EXPERIENCE OF USING THE IMMUNE-COMPLEX VACCINE PULVAK MAGNIPLEX IN THE PREVENTION OF INFECTIOUS BURSA DISEASE OF BROILERS

Abstract

The article presents the results of serological control of the effectiveness of vaccination of broilers against infectious bursal disease (IBD) when the immune-complex vaccine Pulvak MagniPlex is used in the incubator.
 Immune-complex vaccines are vaccines of a new generation, the use of which completely eliminates post-vaccination complications in birds and interference phenomena. The vaccine includes a mixture of specific antibodies and the vaccine strain of the IBH virus. The main property of these vaccines is that when administered subcutaneously in day-old chicks or 18-day-old chicken embryos by the in ovo method, immunity is quickly created even in the presence of high maternal antibodies (MAB). Vaccination in the incubator using immune-complex vaccines is more accurate and provides protection to the whole bird, unlike live vaccines, the main method of which is administration with drinking water. Immuno-complex vaccines are effective even in the presence of high MAT in chickens. Due to this, they provide a much higher level of protection due to the high homogeneity of protective antibodies compared to live attenuated vaccines.
 We present the data of serological monitoring on the average titer of antibodies to the IBH virus in blood sera from 15 batches of broilers aged 45-50 days, which were immunized on the first day in the incubator with the immune-complex vaccine Pulvak MagniPlex. It was established that during a single vaccination in the incubator (15 batches), the Pulvak MagniPlex vaccine stimulates the formation of an active immune response to the IBH virus for the entire period of fattening of broilers. The average titers in the blood sera of broilers aged 45-50 days to the IBH virus ranged from 6,995 to 12,388 after a single vaccination in the incubator (15 batches). The percentage of protective antibodies to the IBH virus in 15 batches of broilers varied from 77.3 to 100, which indicates the intensity of immunity to IBH. A single application of the immune-complex vaccine Pulvak MagniPlex in the incubator stimulates the formation of an active immune response to the IBD virus for the entire period of broiler fattening and provides 100 % protection of 12 batches of broilers and 77.3 % of broilers of 3 batches to IBD. The use of Pulvak MagniPlex allows: to create stable immunity against IBD; to form immunity in each individual broiler, regardless of the level of maternal antibodies; to reduce the impact of stress (compared to mass vaccination in the poultry house by the drinking method. Constant control of the effectiveness of the Pulvak MagniPlex immune-complex vaccine by means of serological monitoring of blood sera in ELISA will allow, depending on the epizootic situation, to timely adjust the vaccination scheme of poultry against IBD.