Abstract
Materials and methods Preliminary tests in disposable-spinners (100 mL culture volume, Corning) were used to determine two suitable media and MC-types for serum reduced expansions (< 10%) of human adipose tissue-derived stem cells (hADSCs; passage 2, Lonza). Using such optimized media-MC-combinations, hADSCs expanded 30 to 40fold, which compares well with expansion rates in planar culture. Based on computational fluid dynamics simulations and suspension analyses in spinners [1], optimal operating parameters were determined in a BIOSTAT UniVessel SU 2 L (2 L culture volume, Sartorius Stedim Biotech).
Highlights
The need for human mesenchymal stem cells has increased enormously in recent years due to their important therapeutic potential
Materials and methods Preliminary tests in disposable-spinners (100 mL culture volume, Corning) were used to determine two suitable media and MC-types for serum reduced expansions (< 10%) of human adipose tissue-derived stem cells
Based on computational fluid dynamics simulations and suspension analyses in spinners [1], optimal operating parameters were determined in a BIOSTAT® UniVessel® SU 2 L (2 L culture volume, Sartorius Stedim Biotech)
Summary
The need for human mesenchymal stem cells (hMSCs) has increased enormously in recent years due to their important therapeutic potential. Expansion of mesenchymal adipose-tissue derived stem cells in a stirred single-use bioreactor under low-serum conditions Carmen Schirmaier1*, Stephan C Kaiser1, Valentin Jossen1, Silke Brill2, Frank Jüngerkes2, Christian van den Bos2, Dieter Eibl1, Regine Eibl1
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