Abstract
Summary. Cord blood (CB) transplantation is primarilyperformed in children, rather than in adults, due to thelow number of haemopoietic progenitor cells obtained fromthe small volume of a single CB collection. Prolongedthrombocytopenia is a major problem following CB trans-plantation. Efforts are currently underway to expand thenumber of CB progenitor cells ex vivo , in order to enabletransplantation in adults and to decrease the period ofthrombocytopenia. In this study we investigated differenttechniques for enrichment and expansion of megakaryocyte(Mk) progenitor cells and haemopoietic stem cells fromCB. CBs from 20 normal deliveries were depleted of redblood cells (RBC) by dividing each sample and testing cellseparation on 3% gelatin, Hespan, Ficoll-Paque or a two-step 3% gelatin followed by Ficoll-Paque separation. Thetwo-step procedure was found to be superior to the othermethods in enrichment of the Mk progenitor cells (CFU-Mk) (34·3-fold), while at the same time retaining thenumber of myeloid and erythroid progenitors, CD34
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
