Abstract
Objective To expand hematopoietic stem/progenitor cells of cord blood in large scale by bioreactor. Methods Mononuclear cells from human umbilical cord blood were cultured in serum-free medium with stem cell factor (SCF), flt-3 ligand (FL3) and thrombopoietin (TPO). The expansion fold of cells, colony-forming and expression of surface molecules were analyzed by cell counting, colony-forming assay and flow cytometry, respectively. And the engraftment of these expanded cells was studied through cell transplantation into irradiated non-obese diabetic/severe-combined immunodeficient (NOD/SCID) mice. Results After culture for 7 days,the folds of total cell expansion in bioreactor were higher than those in static culture, P 0.05). The cells expanded in bioreactor were successfully engrafted into irradiated NOD/SCID mice and reconstructed the multi-lineage hematopoiesis. Conclusion The bioreactor favors large-scale expansion of hematopoietic progenitor cells and keeps the hematopoietic repopulation potential. Key words: Cord blood stem cell transplantation; Cell culture techniques; Bioreactors; Mice, SCID
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
