Abstract
Primary adherent cell types can be expanded in the Quantum® Cell Expansion System (Quantum system), an automated platform that utilizes a hollow-fiber bioreactor. This system can replace manual cell culture and produce cells that retain their phenotypes and functionality. Bone- marrow-derived and adipose-derived mesenchymal stem/stromal cells have previously been successfully expanded on the Quantum system. We have now successfully used the Quantum system to expand fibroblasts and myoblasts. Hollow-fiber bioreactors were coated with adherence-supporting proteins, and then cells were loaded and expanded in the appropriate growth medium for 7 to 15 d. Cells were harvested from the bioreactors using enzymatic reagents. Harvested cell yields ranged from 100 × 106 to 1 × 109 cells, with viability typically above 90%. The number of doublings obtained from Quantum system harvests ranged from 4 to 9. The Quantum system is a functionally closed expansion system that can reduce contamination due to minimal interventions and can automate the culture process to reduce labor and reagent costs.
Highlights
Advances in regenerative medicine have shown that tissues and organs that are impaired by disease, injury, or age can be repaired using cellular therapy [1]
Primary adherent cells (PACs) such as bone-marrow-derived mesenchymal stem cells (BM-MSCs, known as mesenchymal stromal cells or medicinal signaling cells) and adipose-derived mesenchymal stem cells (A-MSCs) have multipotent properties that make them ideal candidates in tissue regeneration [2]
These PACs—bone-marrow-derived mesenchymal stem/stromal cells (BM-MSCs), A-MSCs, fibroblasts, and Skeletal muscle myoblasts (SkMMs)—all have the potential to undergo differentiation into functional cells that contribute to structural regeneration of tissues
Summary
Advances in regenerative medicine have shown that tissues and organs that are impaired by disease, injury, or age can be repaired using cellular therapy [1]. Skeletal muscle myoblasts (SkMMs) are another source of PACs that comprise renewable progenitor cells that participate in injury repair [4] These PACs—BM-MSCs, A-MSCs, fibroblasts, and SkMMs—all have the potential to undergo differentiation into functional cells that contribute to structural regeneration of tissues. The large numbers of ex vivo expanded cells that are required in many clinical cell therapy protocols make standard culture conditions (manual growth in low-volume cell culture flasks or plates) problematic and expensive because of the need for extensive personnel and facility resources and the potential for contamination [5] To meet such clinical demand, a robust, automated, and closed cell expansion method is optimal. Details of the Quantum system’s features and its advantages over manual cell culture processes have been highlighted in a recent review [7]
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