Expanding the Genetic Code in a Mammalian Cell Line by the Introduction of Four‐Base Codon/Anticodon Pairs

Abstract

Switching code at Tyr66. We report the expansion of the genetic code in a mammalian cell line by using various four-base codons. These were derived either from an amber stop codon or rare codons from mammalian cells. Among the four-base codon/anticodon pairs examined, the UAGG/ccua pair was the most efficiently decoded and expressed the highest amount of enhanced green fluorescent protein (EGFP; see scheme). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2006/z500360_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.