Abstract
Exosomes are small extracellular vesicles containing nucleic acid and protein, which have shown a great potential for cancer diagnostics and therapeutic applications. Characterization of exosomes is challenging due to their inherent heterogeneity and complexity. A fractionation step is necessary to provide narrow-sized fractions to enable a more accurate subsequent size analysis. Asymmetrical Flow Field-Flow Fractionation (AsFlFFF) is a high resolution elution technique for fractionation of macromolecules and biological nanoparticles based on their hydrodynamic sizes. In this study the AsFlFFF system was interfaced with Multi Angle Static Light Scattering (MALS) detector to characterize the MCF-7 tumor exosome sample. The analysis revealed a size distribution between 30-120 nm in diameter. Several narrow fractions were collected along the size distribution and were analyzed by PCR. The study showed that the population of tumor exosomes in circulation are heterogeneous in their cancer biomarker miR21.
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