Exosomes Derived from miR‐195‐5p Transfected Bone Marrow Mesenchymal Stem Cells Alleviate Pelvic Floor Dysfunction in Rats

Abstract

AbstractBone marrow‐derived mesenchymal stem cells (BMSCs) can alleviate pelvic floor dysfunction (PFD) in rats. Whether exosomes derived from miR‐195‐5p transfected BMSCs can alleviate PFD is investigated. The rat vaginal distention model is constructed to mimic PFD. miR‐195‐5p is transfected into BMSCs, and exosomes derived from miR‐195‐5p transfected BMSCs are injected into PFD rats via caudal veins. Luciferase reporter assay is performed to confirm the direct interaction between SMAD7 3′‐UTR and miR‐195‐5p mimics. Void volume, bladder void pressure, leak point pressure, and peak bladder pressure are used to evaluate urodynamic function. It is found that miR‐195‐5p directly targets Smad7 and regulates the relative Smad7 expression in rat BMSCs. Exosomes derived from miR‐195‐5p transfected BMSCs can improve urodynamic function than exosomes derived from BMSCs. On the other hand, exosomes derived from miR‐195‐5p transfected BMSCs inhibit the expressions of Smad7 but promote the expressions of elastin in PFD rats with diminished expression of MMP2 and MMP9. This study demonstrates that exosomes derived from miR‐195‐5p genetically engineered BMSCs can target the Tgf‐β/Smad7 pathway to repair connective tissues and restore damaged urodynamic function in PFD rats.