Abstract
Aim: To investigate exosomes as a noninvasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro. Results:Internalized exosomes were detected within 10min of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-h of exposure to exosomes, there were no significant deleterious effects with regardto sperm motility, viability, membrane integrity and mitochondrial membrane potential (p >0.05), thus indicating that exosomes did not interfere with basic sperm function. Conclusion: HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility.
Highlights
The present study shows that HEK293T cell-derived exosomes did not cause deleterious effect on any of the key sperm parameters studied; collectively, these factors are informative of the ability of sperm to undergo in vitro capacitation
We believe that the development of exosome-based delivery systems may provide us with a highly useful tool to develop new ways of studying sperm biology and allow us new options for the diagnosis and treatment of male fertility
Summary
To investigate exosomes as a noninvasive delivery tool for mammalian sperm. We aimed to develop an alternative mammalian-based manufacturing system with scalable applications, for the isolation and exploitation of exosomes as a noninvasive approach for mammalian sperm.
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